謝易修陳霈霓Yih-Shou HsiehPei-Ni Chen蔡淳妙Chun-Miao Tsai生化暨生物科技研究所2026-10-299999-12-312014https://handle.ncl.edu.tw/11296/k99hfqhttps://scholars.csmu.edu.tw/handle/123456789/36006台灣欒樹 (Koelreuteria formosana) 屬無患子科，欒樹屬，為台灣原生種。KFE為台灣欒樹萃取物，是由台灣欒樹的葉部汁液所萃取而得。蛋白酶抑制劑與癌細胞侵襲、轉移有很大的關係，台灣欒樹種子純化出胰蛋白酶抑制劑 (Koelreuteria formosana trypsin inhibitor)，對大腸直腸癌細胞造成細胞凋亡。根據過去文獻，KFE 具有抗氧化，並且能抑制人類癌細胞株的生長。然而，KFE 對人類大腸癌細胞的機轉尚未被研究。本研究的目地為探討 KFE 對人類結腸癌 (DLD-1) 細胞生長存活之影響，並研究其作用機制。在本篇研究中，我們證實了台灣欒樹萃取物 (KFE) 對人類結腸癌細胞株(DLD-1) 引發了強烈的毒殺作用，隨著處理藥物濃度增加，細胞的毒殺性也隨之增加。KFE 處理 DLD-1 細胞後，cleaved-caspase 8 及 9 的蛋白表現量不會有變化，證明 KFE 不會誘導細胞凋亡。另一方面，KFE 處理 DLD-1 細胞後，利用 MDC 及 AVO stain assay，觀察到自噬囊泡 (autolysosome) 的增加，此外，這些細胞自噬的特定標記物， LC3B-II、ATG-3 及 ATG-16 蛋白表現量也明顯增加，而 Bcl-2、PI3K、Akt 和 mTOR 這些細胞自噬與細胞凋亡相關蛋白的表現量，受到 KFE 處理後下降。綜觀上述研究結果，KFE 透過誘導 DLD-1 產生細胞自噬這種抗腫瘤途徑的機轉引發結腸癌細胞死亡。綜合以上，欒樹萃取物誘導結腸癌細胞死亡的機轉，在未來或許可應用在植物化學預防及輔助性癌症治療。Koelreuteria formosana extract (KFE) is a phytochemical compound extracted from the plant, one in medicine is commonly use herbs. Recent studies reported that KFE exhibited inhibitory effects on cell proliferation, migration, and tumor angiogenesis in various cancer cell lines. However, the effects of KFE on anti-cancer properties in colon cell carcinoma (DLD-1) remain unclear. In this study, we demonstrated that the KFE induced a strong cytotoxic effect toward a highly malignant colon cell carcinoma cell line, DLD-1. The mechanisms of the cytotoxic effect were concentration-dependent. On the other hand, these cells showed increased levels of autophagic vacuoles and LC3B-II, ATG-16 and ATG-3 protein expression, specific markers of autophagy. The levels of Bcl-2, PI3K, Akt, and mTOR, which have been implicated in the down-regulation of autophagy and induction of apoptosis, were decreased upon KFE treatment. Taken together, these findings indicate that KFE induced cell death in colon cancer cells via to distinct antineoplastic activities, the ability to induce autophagocytosis, and KFE represent promising candidates for future phytochemical-based mechanistic pathway-targeted cancer prevention strategies.thesis