2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38747Incidence of leukemia is approximately 2-4 persons (per 100,000 populations) a year. The data are the similar with global’s statistics. In 2005, the mortality rate of leukemia was 2.12% on total cancer patient by DOH. Leukemia has ranked the tenth causes of cancer death in adult but the first in children. Cause of leukemia is complicated and the detail mechanism is still unclear. At present, the major treatment of leukemia is use chemistry way, but has strong side-effect. Hence, there is not preventing manner to majority leukemia patient yet. In spite of immense efforts to improve treatment and find cures for advanced diseases, overall mortality rate for most forms of epithelial cancer have not declined in the past 25 years (1). Over the past decade, advances in understanding carcinogenesis have made possible the identification of the candidates of chemoprevention drugs that are being developed to hit the key molecular targets (2, 3). Because of their expected safety for long-term administration to healthy people, current research is in high interest in the diet-derived chemoprevention agents. For example, a mixture of curcuminoids, tea polyphenol extract and broccoli compounds are being developed (4). Cancer prevention has been a world trend. Because of this, searching for healthy food is a very important thing. Our laboratory explores anthocyanins extracted from Hibiscus sabdariffa Linne, Hibiscus anthocyanins (HAs), have antioxidant activity and liver protection. Our previous explored the anti-carcinogenesis effect of anthocyanins and utilized an animal model of N-nitrosomethylurea-induced rats’ leukemia. Based on the data of blood analysis, MPO stain, and death rate, we demonstrate that rat leukemia markedly induced by N-nitrosomethylurea (figure 1, 2 and table 1). These data have been published in Toxicol. Appl. Pharmacol. Therefore, this project will establish the evalution method of health food on the reduction of incidence in human leukemia and estimate the antileukemia possibility of HAs. To divide into three parts: first, we using flow cytometery, western blot, DNA fragment, and MTT assay to analyze the effect; second, we utilize the rat model for the inhibition of leukemia induced by NMU. In addition, we add a clinical drug that the therapeutic application of ATRA (all-trans retinotic acid) to acute promyelocytic leukemia (APL) compares to HAs; third, in the WEHI-3/BALB/c leukemia mice model, the animals were induced by injecting marine WEHI-3 cells and allowed 28 days to develop leukemia. In our previous study, we have proved Hibiscus sabdariffa extract possessing the ability to promote leukemia cell apoptosis and prevente or delay leukemia progression. Herein, we want to setup a series of evaluating methods for health caring foods on preventing or decelerating leukemia. Furthermore, we will also use Hibiscus sabdariffa extract comparing with ATRA to prove the possibility on delaying leukemia.血癌的發生率每年每十萬人口約為2-4人，全世界的統計數據雷同。死亡率方面，根據行政院衛生署民國94年的統計，血癌的死亡人數，佔全部癌症死亡人數的2.12%，佔十大癌症死亡率排行榜之第10名，在兒童惡性腫瘤中，血癌更位居首位。血癌的成因複雜，就至今醫學發展而言，有些致癌機轉的細節，我們仍然不十分清楚，且血癌不像其他的癌症屬固態瘤，故治療有其困難度，雖然目前治療方式主要以全身性的化學治療為主，但會引發病人強烈的副作用，對於大多數的血癌，迄今尚沒有預防之道。美國科學雜誌指出（1），過去二十五年來雖然，在癌症研究方面持續的努力，但對於癌症治療即發生率仍然進展有限，而很多年來化學防癌的表現及角色則給予肯定（2, 3），由於顧慮這些防癌物質的安全性，可食用之天然物質成份mixtures為最好來源，如curcuminoids, polyphenol extracts及broccoli compounds等（4）已被廣泛應用為chemopreventive agents，由於chemporevention的重要性，癌症預防已為一世界潮流，因此，尋找一保健食品（healthy food）為一重要工作。本研究室先前研究，動物模式為使用N-nitroso-N-methylurea（NMU）誘發Sprague-Dawley大白鼠血癌。根據血液分析、骨髓性過氧化酶染色、死亡率統計分析等實驗方法，結果發現NMU可以成功誘導老鼠罹患血癌（附圖一及二、附表一）。本計畫擬進一步利用此模式，建立保健食品降低血癌發生率之評估方法，進而研究洛神花萃取物(已發表於Toxicol.Appl.Pharmacol.具有抑制血癌細胞之作用)是否能作為預防或延緩血癌之保健食品。本計畫使用的研究方法分為三部份，第一：利用細胞實驗（HL-60），以flow cytometery、western blot、DNA fragment及MTT assay等方法分析；第二：利用動物模式，處理全反式維生素A酸（all-trans retinotic acid，ATRA）與洛神花萃取物，研究其降低血癌之作用；第三，利用inoculation方式，以腹腔注射WEHI-3於BALB/c小鼠體內，28天後成功地誘導小鼠產生血癌，探討抑癌之作用。 由於本實驗室已初步證實NMU可成功誘發老鼠罹患血癌，我們在此擬進一步的利用血癌細胞及動物實驗模式，建立保健食品是否能作為預防或延緩罹患血癌之評估方法，並以洛神花萃取物及臨床藥物ATRA證實其在延緩血癌行程評估模式之可行性。保健食品洛神花血癌評估方法Healthy foodHibiscus sabdariffa Linne (Malvaceae)LeukemiaEvaluation methodProject