2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37928Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging offers a modality for simultaneously the spatial distribution of different surface species and the routine analysis of frozen-hydrated biological sample is a goal that is highly sought in the TOF-SIMS community. In this proposal, complete cold chain freeze-fracture sample pretreatment techniques will be developed to test the feasibility of using TOF-SIMS imaging for the heavy metal ions in frozen hydrate liver cells sample. Liver cells will be immersed in solutions of six different heavy metal ions with various concentrations and time durations. Reliable data library associated with the metal intoxication of human liver cells will be created. We will quantify the metal concentrations inside those cells along with depth profile of the liver cell. This study can also provide a chance to understand the time evolution of the damage to the human liver cells caused by heavy metals.飛行時間二次離子質譜儀(TOF-SIMS)離子影像可提供良好的側向解析度，以冷 凍-脆裂法(Freeze-Fracture)將細胞固定於基材上對單一細胞進行分析，是目前飛行 時間二次離子質譜儀對生物組織化學相關資訊研究的重要課題。在本研究計畫裡， 我們將建立一套完整的冷凍-脆裂法生物樣品前處理技術，以提高飛行時間二次離 子質譜儀的離子影像側向解析度。在研究中我們將人體肝細胞分別浸入六種不同種 類及不同濃度的金屬離子溶液中模擬人體肝細胞的重金屬中毒，然後以快速冷凍－ 水合之前處理方法對該重金屬中毒的人體肝細胞進行樣品前處理後再進行二次離 子質譜影像分析。透過這些可靠的樣品前處理技術及二次離子質譜影像分析，我們 將可建立人體肝細胞重金屬中毒的離子質譜影像資料庫；此外，我們也將對重金屬 中毒的人體肝細胞進行縱深分析(dynamic-SIMS)，以瞭解人體肝細胞被重金屬破壞 的過程。Project