JIUNN-LIANG KO2026-10-162026-10-161997-01-01Pure ID: 14910769Pure UUID: 946c5622-3ebf-43c7-822c-11dbdf29ea0510.1023/B:CBTO.0000010392.62109.2chttps://www.scopus.com/pages/publications/0031017712https://scholars.csmu.edu.tw/handle/123456789/29845An abrin-resistant cell line, CHOR 3-4, was isolated from CHOK1 cells which were resistant to a high concentration of abrin (160 ng/ml), and had a 1000-fold higher resistance to abrin than that of CHOK1 cells. CHOR 3-4 cells were about 25-fold more resistant than CHOK1 cells to the N-glycosidase activity of abrin, which was measured by hydrolyzing the N-glycosidic bond of adenine 4324 nucleotide from 3' end of mammalian 28S rRNA. However, the isolated polysomes of CHOR 3-4 cells had the same sensitivity to abrin as those of CHOK1 cells. On measuring the binding of 125I-abrin to CHOR 3-4 cells, it was decreased to about 20% that of CHOK1 cells. This indicates that the mechanism of the resistance of CHOR 3-4 to abrin is due to the alteration of glycoproteins or glycolipids of cell membrane.7enjournal article