2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37654To establish a biomarker for the assay of mobile air pollution, cDNA microarray was employed to compare the gene expression profiles, including those of post-exposure (after continuous working for over a week) and pre-exposure (after a vacation for over a week). A simple chip, which could be used to monitor the impact of engine exhaust exposure on health individuals, was developed through this study. Our preliminary data has shown that up-regulated genes include DNA repair-related genes (such as Ku autoantigen, X-ray repair complementing, excision repair cross-complementing group 2, and AEPX nuclease ), and apoptosis-related genes (such as myeloid cell leukemia sequence1, BCL-2 related, ubiquitin-conjugating enzyme E2B), stress-related genes (such as heat shock 90kD protein and heat shock 27kD protein); down-regulated genes include most of protein synthesis-related genes and ribosomal proteins, for example, ribosomal protein L6, ribosomal protein S4, ribosomal protein S14 and ribosomal protein L5. Based on these results, expression profiles of selected up- and down-regulated genes, 5 for each, from high-exposed cashiers and low-exposed students will be analyzed by RT-PCR to confirm whether the results derived from cDNA microarray can be used as biomarkers of engine exhaust.為建立移動式空氣污染之生物指標，本計劃選取連續工作一周以上之收費員為暴露後之實驗組，同一收費員連續休假一周以上且確定休假期間無其他干擾之污染源為暴露前之實驗組，進行9,600點之生物晶片分析，比較暴露前及暴露後之基因變化並找出較有意義之基因希望能發展出可用來偵測汽車排放物及用來評估暴露者健康效應之簡易生物晶片。初步研究結果如下，促進的基因包括有：與DNA修補有關的基因(如：Ku autoantigen X-ray repair complementing、excision repair cross-complementing group 2、AEPX nuclease)，與細胞凋亡有關的基因(如：Myeloid cell lleukemia sequence1、BCL-2 related、ubiquitin-conjugating enzyme E2B等)，與stress有關的基因 (如：heat shock 90kD protein及heat shock 27kD protein)。抑制的基因則以參與蛋白合成之基因及ribosome蛋白為主，如：ribosomal protein L6、ribosomal protein S4、ribosomal protein S14 及 ribosomal protein L5 等。本計劃將根據以上結果挑選較有意義之促進及抑制之基因各5個針對高暴露的收費員和低暴露之本校學生組以RT-PCR 方法進一步分析基因的變化，以確定生物晶片之分析結果是否能用來作為汽車排放物之暴露生物指標。移動性空氣污染生物指標高暴露組低暴露組cDNA生物晶片Mobile air pollutionBiomarkerHigh-exposedLow-exposedCdna microarayProject