CHENG-MING TANG2026-10-162026-10-162026-06-01Pure ID: 14921494Pure UUID: c1ec452e-fec2-46f7-a706-7d69d8c5379c10.1016/j.mtadv.2026.100822https://www.scopus.com/pages/publications/105039127047https://scholars.csmu.edu.tw/handle/123456789/25052The authors regret for the wrong presentation of figures in Fig. 9 which may need to replace with the correct images. The authors would like to apologise for any inconvenience caused. 1 In Fig. 9.F, the flow cytometry images at A549 cells in control, C-Au, EGFR si and C-Au EGFR si groups were misplaced. However, the semi-quantitative results from the images shown in subfigures are correct. Therefore, the authors have revised Fig. 9F to present the correct images below. [Figure presented] Fig. 9. Assessment for stability of EGFR siRNA conjugated C-Au nanoparticles. Stability of collagen gold, particularly with EGFR siRNA conjugation, may raise concern. We therefore stored C-Au-EGFRsi under 4oC for one month before experiment. The characteristic peat at 520 nm was observed by UV-vis for the three gold containing particles (A). Functional group investigation by FTIR showed identical absorption wave pattern with that of fresh gold decorated nanoparticles. Peak at 2931 for C-H group of collagen was observed shifting after gold nanoparticles associated, and further disappeared with EGFRsi conjugated (B). The stored nanoparticles remained the activity to inhibit EGFR expression of A549 cells, observed by immunofluorescent microscopy at 24 and 48 h for both lipofetamine and C-Au carrier (C), with quantification amount of 0.50-fold at 24 h and 0.38-fold at 48 h by lipofetamine carrier, and that was 0.38- and 0.28-fold by C-Au carrier (D). Cell growth was observed to be decreased to 0.69- and 0.57-fold, at the corresponding time interval by lipofetamine carrier, and that was 0.67- and 0.55-fold by C-Au carrier (E). To check apoptotic and deadly cells using PI/Annexn-V, the maintaining activity for stored carriers can be observed inducing apoptosis and death for A549 at 24 h. (F). The quantification amount, by IF, for apoptosis and death induced by lipofetamine carrier was 1.43- and 1.21-fold, compared to that of 1.56 and 1.21-fold by C-Au carrier (G). By FACS determining, the comparable results were 1.36- and 1.32-fold for siRNA loaded lipofetamine, and 1.48-and 1.36-fold for siRNA loaded C-Au (H). For cell cycle influence, the stored carrier shared similar effects as fresh one, with increased sub-G1 populations and slightly decreased populations in S-phase, particularly for CeAu carrier (I).enjournal article