許立松吳崇毓Chong-Yu Wu生化暨生物科技研究所2026-10-299999-12-312014https://handle.ncl.edu.tw/11296/48qz8ghttps://scholars.csmu.edu.tw/handle/123456789/36069Krüppel-like factor 家族成員主要扮演細胞內，調控生物功能之轉活化控者 (transcriptional activators) 以及轉錄抑制者 (transcriptional repressor) 的腳色，本篇所探討的 KLF6、KLF10 便是屬於此一家族的成員，而就先前研究指出 KLF6、KLF10 被歸類為轉錄抑制者，這是由於其基因序列中含有轉錄抑制區域 (repression domains) ，更有文獻指出 KLF6 對於細胞之轉移以及 KLF10 對於細胞增生作用是有影響的，然而 KLF6、KLF10 對於口腔癌及胃癌的影響仍未完全明瞭。在本實驗中我們探討，KLF6 對口腔癌細胞 SAS 遷移之現象的影響，首先經由細胞傷口癒合實驗、細胞移動實驗，證實了 KLF6 過表現的組別與控制組相比確實在細胞遷移的作用上有顯著的差異。另外 KLF10 對胃癌細胞 AGS 生長的影響，以流式細胞儀進行細胞週期分析發現 KLF10 過表現造成細胞凋亡現象，進一步以西方點墨法證實 KLF10 確實影響了 bcl-2 及 caspase-3 蛋白的表現，再透過二維電泳分析鑑定出 ARP3、XRCC6、aldose reductase、protein phosphatase 1 的在 AGS 胃癌細胞中參與了 KLF10 的作用。KLF6 and KLF10 belongs among the Krüppel-like factor factor family members, this family members play a major role in regulation of biological functions as transcriptional activators or transcriptional repressors. Reports have been shown that KLF6 and KLF10 are classified as transcriptional repressors, due to their genetic sequences comprising repression domains, Previous reports have demonstrated that KLF6 involved in cancer migration whereas KLF10 participitated in cell proliferation. However, the effects of KLF6 and KLF10 in oral and gastric cancer are not fully understood. Overexpression of KLF6 in oral cancer SAS cells enhanced migration as evidenced by the wound healing assay and cell migration assay. In addition, KLF10 triggered apoptosis in gastric cancer AGS cells as measured by flow cytometry. Western blot analysis indicated that KLF10 affect the expresion of bcl-2 and caspase-3 protein. Through the two-dimensional electrophoresis analysis identified ARP3, XRCC6, aldose reductase, protein phosphatase-1 involved in KLF10 signals in AGS gastric cancer cells.thesis