2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38542Most of nursing-home residents suffer from constipation. I have investigated the laxative effect of fructo-oligosaccharide and isomaltooligosaccharide in the constipated nursing-home residents and found each supplement effectively improved the bowel habit in this population. However, it has not been investigated whether these two oligosaccharides modulate colonic microflora and risk factors of colon cancer in constipated nursing-home residents. On the other hand, biological effects of oligosaccharides are thought to be mediated by its fermentation products and the probiotics. Previous animal studies have found supplementation of probiotics promoted the blood anti-oxidative status in rats, which has not been investigated in men. Therefore, I plan to investigate effects of fructo-oligosaccharide or isomaltooligosaccharide on the colonic microflora, blood anti-oxidative status and risk factors of human colon cancer in constipated nursing-home residents during the following three years. During 2007-2008, we will recruit constipated nursing-home residents (20 volunteers for each study) to participate this single-blind, placebo-controlled, self-comparison, sequential study. Each study includes placebo (14-d), experimental 1 (28 d), experimental 2 (28 d) and withdraw (28 d) periods. Fructo-oligosaccharide or isomalto-oligosaccharide (10 g active compounds) will be given as a drink in the afternoon to the volunteers during the experimental periods. We will collect feces during the last week and fasting blood on the last d of each period, in order to analyze the bowel habit, fecal characteristics, colonic microflora, plasma antioxidant concentrations, blood anti-oxidative enzyme activities such as glutathione peroxidase, glutathione reductase, superoxide dismutase, and plasma lipid-peroxidative product concentrations. In 2009, we will determine the anti-oxidative status of fecal water. In addition, we will determine the colon-cancer-related risk factors, such as fecal bacterial enzyme activities, and bile acid concentrations in the fecal water. Furthermore, we will investigate whether fructo-oligosaccharide or isomalto-oligosaccharide modulates the genotoxicity of fecal water on the Caco-2 human carcinoma cells, and the resistance of Caco-2 human carcinoma cells to the H2O2-induced oxidative stress. Results from this 3-year research will hopefully support the use of oligosaccharides on the constipated nursing-home residents as an anti-aging and colon-cancer-prevention food.安養院居民慣性便秘的比例甚高，本實驗室已探討並發現補充果寡醣 及異麥芽寡醣皆可顯著改善慣性便秘安養院居民之排便習慣，但是尚未探 討果寡醣及異麥芽寡醣對於這些居民腸道菌相及大腸癌前危險指標的影 響。再者，寡醣的生理功能常藉由其發酵產物及刺激益生菌的生長而產 生，而益生菌已被發現有提高血液抗氧化的作用，然而目前尚未有相關的 人體實驗驗證寡醣對體內氧化狀態的影響。因此，申請人提出三年研究計 畫： 於96-97 年進行人體實驗，主要探討果寡醣或異麥芽寡醣對慣性便秘 安養中心居民大腸菌相、短鏈脂肪酸及血液抗氧化狀態之影響。於98 年 則延續前兩年的研究，主要目的是探討果寡醣或異麥芽寡醣對慣性便秘安 養中心居民大腸癌危險指標、糞便水膽酸、糞便水抗氧化性及糞便水對大 腸表皮細胞基因毒性之調節作用。 本提案將於96、97 年經由家醫科主任醫師徵求安養院居民(每一年20 名)，進行單盲、自我對照實驗，實驗分為控制期(14 天)、介入期一(28 天)、 介入期二(28 天)及淨空期(28 天)四期，實驗中參試者於控制期攝取果糖水 作為安慰劑，介入期一的前7 天作為適應期，攝取只含5 克活性寡醣成分 的水，之後直到介入期二結束都攝取含10 克活性寡醣成分的水。每一期最 後7 天收集所有糞便，冷凍乾燥後避光保存；最後一天收取禁食血，適當 前處理後保存於-80℃。 我們將於96、97 年分析: (1)排便狀況；(2)糞便特性及短鏈脂肪酸；(3) 腸道菌相: 以螢光原位雜交法定量總菌數、Bifidobacterium、Lactobacilus、 Bacteroides、Clostridium 及E. coli；(4) 血液抗氧化力: 包括血球彗星電泳 實驗、血漿維生素C、E、glutathione 濃度、血液抗氧化酵素glutathione peroxidase, glutathione reductase, superoxide dismutase 活性；(5)血液脂質過 氧化產物；(6)血液營養生化指標、肝腎功能指標；(7)基本個人資料、基本 體位、營養素攝取等資料。98 年將分析先前取得糞便樣品之: (1)大腸癌相 關之細菌酵素，包括芻-D-galactosidase、芻-glucosidase、芻-glucuronidase、 mucinase; (2)糞便水各式一級及二級膽酸濃度；(3)糞便水抗氧化力，包括 螫合鐵Fe2+能力、共軛雙烯化合物產生之抑制率及DPPH 自由基清除率。 我們亦將以大腸腺癌細胞株(Caco-2)作為大腸表皮細胞模式，運用彗星電泳 實驗來探討寡醣對參試者糞便水在Caco-2 細胞上的基因毒性表現，以及寡 醣是否可以抵禦H2O2 對Caco-2 細胞造成的氧化壓力。 申請人希望藉由此研究做為寡醣成為老人優良膳食補充食品的科學依 據，並提升我國老人醫學領域的表現。Project