YAN-ZIN CHANG2026-10-162026-10-162009-02-15Pure ID: 15190128Pure UUID: 3a2058dd-0903-4c73-b0d4-856c0cb0a15410.1016/j.talanta.2008.09.017https://www.scopus.com/pages/publications/57249097124https://scholars.csmu.edu.tw/handle/123456789/25963An integrated method of liquid chromatography-heated electrospray ionization/tandem mass spectrometry was evaluated for high throughput screening of various abused drugs in urine. Chromatographic analysis was performed on a C18 reverse phase column using a linear gradient of 10 mM ammonium acetate containing 0.1% formic acid-methanol as mobile phase and the total separation time was 7 min. A simple and rapid sample preparation method used was by passing urine samples through a 0.22 μm PVDF syringe filter. The detection limits of the studied abused drugs in urine were from 0.6 ng mL-1 (ketamine) to 9.0 ng mL-1 (norcodeine). According to the results, the linear range was from 1 to 1200 ng mL-1 with relative standard deviation (R.S.D.s) value below 14.8% (intra-day) and 24.6% (inter-day). The feasibility of applying the proposed method to determine various abused drugs in real samples was examined by analyzing urine samples from drug-abused suspects. The abused drugs including ketamines and amphetamines were detected in suspected urine samples. The results demonstrate the suitability of LC-HESI-MS/MS for high throughput screening of the various abused drugs in urine.7enjournal article