許閔彥黃建寧Min-Yen HsuChien-Ning Huang李致名Chee-Ming Lee醫學研究所2026-10-292022-08-122022https://handle.ncl.edu.tw/11296/9vk945https://scholars.csmu.edu.tw/handle/123456789/36652研究目的：Growth arrest-specific 5 (GAS5) 是一個與細胞分裂、分化、免疫、以及血管新生有關的長鏈非編碼核糖核酸(long Non-Coding RNA, lncRNA)，並且與癌症、癌症的血管新生、糖尿病有關。糖尿病視網膜病變是一個與血管內皮新生因子(vascular endothelial growth factor, VEGF)及新生血管相關的疾病。我們希望藉由糖尿病患者的基因分析來探討GAS5基因變異與糖尿病視網膜病變的相關性。研究方法及資料：本研究採前瞻性病例對照研究。我們的研究納入了中山醫學大學附設醫院722位糖尿病患，並取得其血液檢體，進行核酸萃取，使用即時聚合酶連鎖反應進行GAS5 單一核苷酸多型性(SNPs)包括 rs145204276 (Ins/Del) 及rs55829688 (T/C)的分析。本研究經資料蒐集完成後，以SAS 9.4版軟體進行“獨立樣本檢定”(independent tests)、“多元邏輯斯迴歸分析”(multiple logistic regression analysis) 、“次群組分析”(subgroup analysis) 、及“杜凱事後比較檢定”(Tukey's a posteriori comparison) 。研究結果：我們發現在糖尿病患者中，糖尿病視網膜病變病患(DR Group) GAS5 單一核苷酸多型性(SNP) rs145204276 del/del的比例比無糖尿病視網膜病變病患(Non-DR Group)顯著較高。而在次群組分析中，非增殖性糖尿病視網膜病變病患(NPDR Subgroup) 在GAS5 rs145204276 del/del的比例也比Non-DR Group顯著較高。而增殖性糖尿病視網膜病變病患(PDR Subgroup)在GAS5 rs145204276 del/del的比例與Non-DR Group沒有顯著差異。結論：GAS5 SNP rs145204276 del/del可能與糖尿病患者發生糖尿病視網膜病變相關。Objective: Growth arrest-specific 5 (GAS5) is a long non-coding ribonucleic acid (lncRNA) associated with cell division, differentiation, immunity, and neovascularization, and is associated with cancer, cancer neovascularization, and diabetes. We aimed to investigate the association between GAS5 genetic polymorphism and diabetic retinopathy by genetic analysis of diabetic patients.Methods and Materials: This study was a prospective case-control study. We collected 722 diabetic patients from Chung Shan Medical University Hospital and obtained their blood samples for nucleic acid extraction, and used Real-time PCR to analyze the single nucleotide polymorphisms (SNPs) of GAS5 including rs145204276 (Ins/Del) and rs55829688 (T/C). After data collection, independent tests, multiple logistic regression analysis, subgroup analysis, and Tukey's a posteriori comparison were performed using SAS version 9.4 software. Results: We found that the proportion of GAS5 SNPs rs145204276 del/del was significantly higher in patients with diabetic retinopathy (DR group) than in patients without diabetic retinopathy (Non-DR Group). In the subgroup analysis of diabetic retinopathy, the same results were found for non-proliferative diabetic retinopathy patients (NPDR Subgroup) compared with Non-DR Group, but there was no significant difference in patients with proliferative diabetic retinopathy (PDR Subgroup) compared with Non-DR Group.Conclusion: Single nucleotide polymorphism of GAS5 rs145204276 del/del may be associated with the development of diabetic retinopathy in diabetic patient.Growth arrest-specific 5基因變異糖尿病視網膜病變糖尿病病程時間genetic variantsdiabetic retinopathydiabetes mellitusdurationthesis