2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38802Cancer prevention has been a world trend. Because of this, searching for chemopreventive agents is a very important thing. Anthocyanins are abundant in vegetables and fruits. Many reports indicated its have function in anti-oxidation, reducing cholesterol, and preventing cancers. Our laboratory explores anthocyanins extracted from Hibiscus sabdariffa Linne, Hibiscus anthocyanins (HAs), have antioxidant activity and liver protection. Previously, we found that HAs have anti-carcinogenesis effect in vivo and in vito. Therefore, we further work are study that the mechanism of HAs inhibits leukemia effect. First year, we utilize the same rat model for the inhibition of leukemia induced by NMU. Furthermore, to determine N-Nitroso-N-Methylurea (NMU)-induced Sprague-Dawley rat leukemia is classifies to leukemia M3 (APL, acute promyelocytic leukemia). In addition, we treat with ATRA (all-trans retinotic acid) that is a clinical drug of APL and the effect will be comparing with HAs. Second year, we employ different animal model (inoculation) to further investigation. WEHI-3 cells inject to BALB/c mices (i.p.), and then leukemia was successfully induced after 28 days. We same add the two different drug (HAs and ATRA) in this research. Results can be confirmed each other with the first year of data. The works are including (1) prepare abundant HAs; (2) animal model progression; (3) blood and biochemistry analysis; (4) histology, ex. H&E stain; (5) IHC, ex. CD markers; (6) RAR/RXR-relative gene and protein expression; (7) apoptotic protein expression, et al. According to these studies, we demonstrate that NMU can induce rats APL. It would also prove HAs have the effect of anticancer and cancer prevention and could contrast the two drugs. The mechanism of repression of animal’s leukemia by HAs has been well characterized. Moreover, we apply IND to progress human clinical test of traditional herb. We expect it could application for developing a new chemopreventive agent.癌症預防已為一世界潮流，因此，尋找一化學預防物質（chemopreventive agent）為一重要工作。花青素（Anthocyanins）廣存於蔬菜、水果等植物中，已有許多報告證明花青素具抗氧化活性，降低血脂肪及防癌等作用，本研究室也發現分離自洛神花之花青素（Hibiscus anthocyanins，HAs）有抗氧化及保肝之功能。先前的工作利用細胞及動物實驗，結果顯示洛神花之HAs具有抑制血癌之作用。因此，往後的工作，我們將深入研究HAs抑制血癌之機制。第一年的工作將完成之動物實驗進行鑑定，確認N-Nitroso-N-Methylurea（NMU）所誘導雄性大白鼠（Sprague-Dawley rat）血癌之動物模式，屬於M3期之急性前骨髓細胞性白血病（APL，acute promyelocytic leukemia）。另外，同時處理全反式維生素A酸（all-trans retinotic acid，ATRA）與洛神花萃取物（HAs），研究並比較兩者其降低血癌之作用。第二年的工作為利用不同之動物模式即inoculation方式，以腹腔注射WEHI-3於BALB/c小鼠體內，28天後成功地誘導小鼠產生血癌，同樣處理HAs及ATRA，探討其抑癌之可能機轉，進一步與第一年之結果相互印證。實驗項目包括：（1）完成誘發血癌之動物實驗；（2）血液及生化分析；（3）周邊血液抹片分析，如劉氏染色、柏氏染色；（4）組織學分析，如H&E染色；（5）免疫組織化學染色（IHC）；（6）探討RA receptor（RAR）或retinoid X receptor（RXR）相關的基因與蛋白表現；（7）探討抑制血癌作用之相關的訊息傳遞路徑等方法。綜合以上，確認NMU所誘發之動物血癌為急性前骨髓細胞性白血病（Acute promyeloid leukemia，APL），並且利用本實驗室所分離之HAs與市售之ATRA，比較兩者抑制血癌之作用，進而釐清其詳細相關分子機制。此動物模式可作為佐證洛神花抗癌之作用，期許未來可推衍於人體，進一步申請IND執行中草藥人體臨床試驗，發展為抗癌新藥。洛神花花青素抑制癌化血癌Hibiscus sabdariffa Linneanthocyaninsanti-carcinogenesisleukemiaProject