2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38626Mineral trioxde aggregate (MTA) material is common used in dental clinic. Our laboratory continue performing the similar projects from National Science Committee for two years. At this moment, the primary results has come out. The self made MTA has better physical property on setting time than commercial MTA. The self made MTA has similar chemical properties as commercial MTA. The cytocompatibility of self made MAT is compatible. The result has submit to journal and in revise. Till now, although the material is cytocompatibile, but there still need to investigate the immune reaction and its biomechanism on cell either in vivo or in vitro. This project is to study following objects in three years. The first year objective is to evaluate the self made MTA material its immune reaction. The second year objective is to evaluate the self made MTA its bone effect and its probably signal transduction pathway. Material and methods: The first and second year is in vitro experiments. The self made MTA is following the previous protocol to make up. Material dissolute in normal saline, dilute to different concentrations. The target cell is using the human osteosarcoma cell line (U2OS) because its similar effect as osteocyte. The IL-1α,1β and IL6 will be evaluated by ELISA method. The activity of osteocalcin, alkalaine phosphatase evaluated too. To detect the cell activity, the ALP activity will be evaluated. By applying the RTPCR method, the COX-2 expression can be discovered. The western blot analysis is to analysis the COX-2 protein、ERKinase、JNK kinase、caspase 3 、alkaline phosphatase, osteonidogen, osteonectin,, osteopontin protein expression. The third year is in vivo experiment. We will select the 60 sprague Dawley male rats as the sample. On rat parietal bone and subcutaneous, the material will be implanted. In this experiment, the hydroxyl apatite is as control group. The inflammation of the implant site will be calculated. All results will be recorded and statistic with statistical software and with p value less than 0.05 showed statistical difference. From present study, it is hope that the self made material can be applied on human in feature.矽鈣化合物應用於牙科之治療非常普及， 本研究室由九十五年至九六年國科會 計畫中陸續執行自行研發合成之三氧礦化物(mineral trioxide aggregate)之物理、 化學性質與體外之細胞生物活性研究。至目前已有部分結果，並已投稿中。由於 本研究室合成之材料其硬化時間較商品化的硬化時間短，且至目前為止，結果顯 示材料對於細胞之生長不具有毒性。然而由於對於生物性材料之使用標準，另需 考慮免疫方面問題與活體上使用之安全與成功率。因此動機上，本計畫期望以三 年時間，將自行研發之材料有機會繼續進行體內與體外之實驗。因此本研究目的： 1. 於體外試驗，探討自行合成之MTA之對細胞發炎免疫方面反應。 2. 於體外試驗，探討自行合成之MTA於細胞內對於骨生成因子之訊息變化。 3. 於體內試驗，探討自行合成之MTA於動物體內植入後之生物體反應。 研究材料與方法： 第一與第二年為體外試驗。依過去的製程MTA燒結出，配製成所需的溶液，細 胞採用U2OS 細胞株。於細胞發炎ELISA分析，以材料作用於細胞後，分析有關 於負責活化骨細胞之細胞素如； IL 1α,1β和IL6之變化。同時也分析osteocalcin， alkalaine phosphatase 之活性。以鹼性磷酸脢(alkaline phosphatase [ALP] activity) 分析材料與細胞作用後之活性。以RTPCR方法，觀測細胞COX-2的表現。以西 方墨點分析探討細胞內訊息蛋白COX-2 protein、ERKinase、JNK kinase、caspase 3 、alkaline phosphatase, osteonidogen, osteonectin, 和osteopontin之的表現，以了 解自行研發材料的生物活性機轉。第三年為作用於老鼠體內的試驗，以60隻 Sprague Dawley rat作為植入之對象，以合成之MTA為實驗組，另外以Hydroxy apatite 作為控制組，作為比較組織反應之差異性。植入部位為parietal bone和 subcutaneous位置，觀察記錄其組織之發炎反應，並紀錄之。所有結果居已統計 軟體分析比較結果。本研究預期於上述之實驗後應可證明材料具生物相容性，或 繼續改良其缺點，以利日後進行人體試驗。三氧礦化合物訊息傳遞發炎反應動物實驗材料相容性mineral trioxide aggregatesignal transductioninflammationanimalstudybiocompatibilityProject