2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38559Neutrophils represent the most massive leukocyte population in peripheral blood and are the most important cell types that participate in early inflammation. During an inflammatory period, margination of neutrophils onto endothelial cells lining on the blood vessel wall is initiated, subsequently, these cells adhere to the endothelial cells tightly in response to local inflammatory signals followed by extravasation through the vessel wall and migrate directionally toward the concentration gradient of chemoattractants, or migrate further through epithelial cells toward epithelium lumen where they perform the phagocytic function. The activation of migration and phagocytosis also activate the destructive mechanisms of neutrophils, and result in resperitory burst and the release of hydrolytic enzymes from the cytoplasmic granules to extracellullar space for killing and hydrolysis of foreign particles such as bacteria and debris. Such activity usually damage normal host cells at the inflammatory site, thus also represent the pathophysiology of various acute and chronic inflammation. Consequently, the inhibition of neutrophil migration and/or the activity thereafter has been suggested an important strategy in development of anti-inflammatory drug. Garlic has been recognized to possess diverse biological activities including anti-inflammatory property; however, the majority studies have been focused on its effect on the activity of macrophages, another mediator/effector in an inflammatory reaction. There is a lack of systemic study on the effect of garlic on the activity of neutrophils in term osf the functional composition of garlic, the consistency of investigation model and the completion of investigation for known neutrophil function. The project applicant and co-workers have reported a variety of biological activities of garlic oil and its organosulfur compounds (OSCs) , including the finding that neutrophil being a target of these garlic compounds in regulate endotoxin-induced intestinal inflammation. The aim of the present study is to investigate systematically with garlic oil and its major organosulfur compounds for their anti-inflammatory effect through the mediation of neutrophil activities. The underlying mechanisms will also be investigated. In particular, the present study is to understand whether garlic oil and its major organosulfur compounds ameliorate endotoxin-induced intestinal damage by affecting adhesion, chemotaxis, phagocytosis and degranulization of neutrophil and the interaction between neutrophils and intestinal epithelial cells.嗜中性球是周邊血液中含量最多的白血球，也是參與發炎反應早期最重要的細胞類 型。在發炎期間，此細胞先著邊於血管壁內皮，繼接受到局部發炎信號後與內皮細胞發 生緊密黏性，並受到發炎部位趨化因子濃度梯度的牽引，產生方向性的遷移，由血管壁 滲出至組織中，並可進一步穿越上皮遷移至上皮空腔內，進行吞噬作用。遷移與吞噬過 程活化細胞的破壞性機制，使細胞發生氧化爆炸及釋放出胞漿顆粒中所含有的各種水解 酵素以利殺菌及分解碎屑。由於這些活性亦傷及發炎部位正常宿主細胞，往往也是許多 急慢性發炎的病理生理學特徵。因而抑制此細胞遷移及破壞性活性，如今已成為研發抗 發炎藥物的重要策略之一。過去已有許多學者提出大蒜具有之多重生物學作用，其中包 括抗發炎作用，但主要係針對發炎反應中另一個調節性/作用性細胞—巨噬細胞，之活 性所進行的觀察。雖然過去曾有少數學者報導大蒜可抑制嗜中性球活性，但不論就大蒜 成份、研究模式或所觀察之嗜中性球活性項目的完整性而言，均缺乏系統性的研究。而 本計畫申請人與同僚過去已報導關於富含有機硫成分(OSCs)之大蒜精油及其含硫衍生 物之許多生物學活性，並發現在內毒素誘發之腸道發炎性損傷中，嗜中性球是大蒜精油 的作用標的之一。本研究的目的係針對大蒜精油及其OSCs 的抗發炎作用是否可透過對 於嗜中性球遷移及活化特性之調控及其相關機制進行系統性探討。而本研究之特殊目標 是瞭解大蒜精油及其OSCs 是否透過影響嗜中球黏附、趨化、吞噬及釋放顆粒作用而提 供抗發炎效果，達成緩和內毒素所致之發炎反應中小腸黏膜損傷之作用，同時瞭解這些 大蒜成份對於嗜中性球與小腸上皮細胞間交互作用之影響。大蒜精油 有機硫成分 發炎 嗜中性球 小腸上皮細胞 大鼠garlic oil--inflammation--intestinal epithelial cells--neutrophil--organosulfurcompounds—ratProject