李宣佑Shuan Yow Li葉必明Pi Ming Yeh醫學研究所2026-10-289999-12-31https://handle.ncl.edu.tw/11296/76tqt5https://scholars.csmu.edu.tw/handle/123456789/34826近年來許多研究顯示,長期飲用含砷的飲水會導致腫瘤的發生;陳等自1985 年研究台灣烏腳病地區居民罹患惡性腫瘤的情形比台灣一般民眾罹患惡性 腫瘤的情形高出許多.於是,在烏腳病盛行地區取樣當地皮膚癌患者(非惡 性黑色素腫瘤且無烏腳病者)32位,及健康居民(無癌症且無烏腳病)32位, 抽取血液進行分析,想進一步利用:1.染色体斷裂頻率及斷裂位置 2.姊妹 染色體交換頻率 3.微小核出現的頻率,來瞭解砷與皮膚癌的相關性.結果 顯示:1.染色體斷裂頻率方面:(1)對照組與皮膚癌患者染色體斷裂數目以 arsenic加 bleomycin組最多,bleomycin組次之,arsenic組第三, untreated組最少;(2)對照組方面:untreated組為2.93個/人 , arsenic組 為7.59個/人,兩組之間有顯著差異(p < 0.01);(3)皮膚癌患者:untreated 組為2.46個/人,arsenic組為6.31個/人,兩組之間在90%顯著水準下有顯著 差異;但在95%下卻沒有顯著差異(p >0.05);(4)ble- omycin 處理方面:對 照組為106.33個/人,皮膚癌患者為178.92個/人 ,兩者之間有顯著差異(p <0.01);(5)arsenic加bleomycin組方面:對照組為145.74個/人,皮膚癌患 者為239.46個/人,兩者之間有顯著差異(p< 0.01);(6)對照組與皮膚癌患 者染色體斷裂數目均顯示:arsenic加ble- omycin組比bleomycin組多, 在90%顯著水準下有顯著差異;但在95%下卻沒有顯著差異.2.染色體斷裂位 置方面:以 arsenic加bleomycin組染色體斷裂頻率最高,平均值超過10個/ 人之染色體部位,對照組為:2q,3q, 4q,5q;皮膚癌患者為:1p,1q,2p,2q,3 p,3q,4q,5q,6q,7q,8q;而對照組與皮膚癌患者皆以染色體4q位置斷裂數目 最多.3.姊妹染色體交換頻率方面:對照組與皮膚癌患者姊妹染色體交換頻 率都隨著加入砷的濃度增加而增加.姊妹染色體細胞生長指數方面兩組都 顯得沒有差異.4.微小核出現的頻率方面:對照組方面untreated組與 arsenic 1uM組之間於 93%顯著水準下有顯著差異.綜合以上的結果,我們 可以瞭解砷對造成皮膚癌確實有不可忽略的重要性,但是,在日常生活中砷 都以微量的狀態存在,而且要長時間慢性的累積才會發病,因此,往往容易 被忽視,本篇研究除了發現砷最常引起染色體斷裂的位置,也進一步以人體 實驗性的方式證實了砷與皮膚癌之間的相關性.Many studies in recent years have shown the occurrence frequency of malignant neoplasms among residents of the blac- kfoot disease endemic area is higher than that of the general population in Taiwan. In this study,we use cytogenetic method as the followings: 1.human lymphocyte chromosomal breakage frequency and sites 2.sister chromatid exchange(SCE)frequence 3.micronuclei(MN) frsequency,to understand the relationship between arsenic and skin cancer.We selected 32 skin cancer patients and healthy residents in the endemic area of high- arsenic artesian well water in Taiwan. The results showed that in chromosomal breakage frequency: 1.The health residents' frequency of chromatid breakage is si- gnificantly higher among arsenic treated than among untreated. But skin cancer patients' frequency of chromatid breakage be- tween untreated and arsenic treated has no significant differ- ence. 2.Bleomycin treatment: Skin cancer patient' frequency of chromatid breakage is significantly higher than the healthy residents'.3.Arsenic & Bleomycin treatment:Skin cancer patien- ts'frequency of chromatid breakage is significantly higher th- an the healthy residents'.The bleomycin can induce chromosomal breakage which appears to be the difference between skin canc- er patients and the healthy residents.In the sites of chromos- omal breakage: The highest frequency of chromatid breakage was found in the group of arsenic & bleomycin treatment.The highe- st chromatid break site is at chromosome 4q for both the heal- th residents and the skin cancer patients.In the frequency of sister chromatid exchange:The frequency of sister chromatid exchange proportionally increase according to the concentrati- on of the arsenic.In the frequency of micronuclei:There is si- gnificant difference between untreated and 1uM arsenic treated skin cancer patients.Therefore,we concluded that there is imp- ortant relationship between arsenic and skin cancer.砷皮膚癌染色體變異ArsenicSkin cancerChromosomal aberrationthesis