2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37454There are a number of functional oligosaccharides in the market, including fructooligosaccharide、isomaltooligosaccharide、galacto-oligosaccharide and soyoligosaccharide. These oligosaccharides are added in various kinds food or food additives. All of these oligosaccharides, more or less, have the attribute of difficult digestibility to increase intestinal tract movement and inhibit the synthesis of lysis products. Furthermore, they have several physioligical functions in animals that effects on gut. There are various microflora have positive in the digestive tract, skin and urinary organs in healthy animals. The microflora and host animals are mutually beneficial to each other. In the gut microflora, the most helpful bacteria to the host are bifidobacterium and lactobacilli. Mean while, because the helpful flora in gut can produce vitamin B1 and B6, by using the digestive enzyme of flora, the nutrition absorption can be advanced. Furthermore, lactate and acetate produced by the flora could inhibit the production of harmful flora in gut. Furthermore, they could improve the recovery from diarrhea、constipation and the high ammoniemia of barriers of liver function obstacle in clinical.In this study, we will use the TSC fructooligosaccharide to feed mice and observe the pattern changes of intestinal microflora, particularly these beneficial flora, such as bifidobacterium、lactobacilli and the anaerobic harmful flora like clostridium. At the same time, we’ll examine the activity of digestive enzyme and observe the gastrointestinal tract movement of the control and experimental group treated with fructooligosaccharide by using the oral intake with activated carbon.目前市面上有多種機能性寡糖，如果寡糖、異麥芽寡糖、半乳寡糖、大豆寡糖等寡糖類食品或食品添加物等的方式存在，這些寡糖類食物或多或少都具有難消化性因素，而使腸管運動增加，腸內腐敗物及抑制對身體有害物質的代謝和整腸的作用，亦是目前極為受到重視的健康食品。健康的動物除了在於消化管道之外，尚有皮膚、泌尿生殖器官等也均有常在的菌叢存在，會與宿主的健康相互影響著。腸內菌叢中以bifidobacterium lactobacilli 是對於宿主最為有益的腸內菌種最令人注意，同時因腸內有益性的菌種亦可產生B1及B6及利用菌的消化酵素也可促使宿主對於營養素的吸收增加，而所生成的乳酸及醋酸可抑制腸內有害菌的增殖。在臨床上亦有改善下痢或便祕及肝機能障礙時所產生的高amonia血症等均有其效果存在。本計劃是利用台糖之果寡糖餵食實驗動物後觀察其腸內菌叢的改變。特別是對於身體有益的菌種如bifidobacterium lactobacilli等以及對於腸內增殖有害的菌種如clostridium等厭氧性菌為主要目的。同時並測定動物體內消化酵素活性及利用活性碳來觀察控制組及添加果寡糖組其胃腸運動的情形。果寡糖比菲德氏菌乳酸桿菌梭狀芽孢桿菌屬FructooligosaccharideBibidobacteriumLactobacilliClostridium BacteriaProject