2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/38697Bone defects occur in a wide variety of clinical situations, and their reconstruction to provide mechanical integrity to the skeleton is a necessary step in the patient’s rehabilitation. To date, a variety of materials such as glass-ionomer cement, bioactive glass cement, calcium phosphate- based cement have been clinically evaluated as replacements for damaged hard tissues. In order to be suitable for bone tissue repair, a synthetic material must be biocompatible and it should exhibit some structural and mechanical equivalence to bone that is a composite material consisting mainly of an organic matrix (collagen) and a mineral phase (hydroxyapatite). Silicon, an important trace element in an early stage of bone formation, increases directly with calcium at relatively low calcium concentrations and falls below the detection limit at compositions approaching hydroxyapatite. The soluble form of Si may stimulate collagen type I synthesis and osteoblastic differentiation. The silicate ions might provide favorable sites for nucleation of the apatite on the Si-containing biomaterials when immersed in simulated body fluid. Hence, calcium silicate cements will open up new possibilities in the field of self-setting bioactive filling materials. The commonly used cements such as calcium phosphate cement (CPC), when implanted as a paste that is just starting to set, tend to disintegrate and washout upon early contact with blood or other fluids. For this reason, a great attention has been paid to the anti-washout-cement that some cohesion promoters were added to prevent this disintegration. To the best of our knowledge, there have been no reports on the development of anti-washout calcium silicate cements with fast setting time and high bioactivity. The present three-year project that combines natural polymer with bioactive calcium silicate attempts to achieve elastic and highly bioactive bone cement with washout-resistant ability. The first-year objective will optimize calcium silicate cements via various characterization methods, in vitro evaluation in physiological solution, and cell biocompatibility monitoring. The processing parameters include sol-gel route such as precursor ration, ageing time, and calcination temperature. In the second year we will study whether the present potential materials could stimulate cell proliferation, differentiation, and gene expression using three different types of cells. Finally, the design of elastonic composite cement with anti-washout property will be achieved. The natural polymers such as chitosan and gelatin will add to the ceramic cement to improve the cohesiveness and working properties of the novel calcium silicate cement system. Combination of basic science and medical practice, results of this three-year research are expected to have a significant potency in the areas of biomaterials science and biotechnology.各種情況下皆會發生骨缺損，病人癒合的必要步驟是經由骨缺損重建以提供骨骼完整機械性。目前臨床上使用玻璃離子體、生物活性玻璃及鈣磷酸鹽骨泥等材料修補骨缺陷，而適合作骨組織修補用的材料必須具備優異生物活性，且與骨組織（主要是由有機相（膠原蛋白）與無機相（氫氧基磷灰石）構成之複合材）相近的結構及機械性。矽是早期骨形成階段的重要微量元素，在低鈣濃度時其含量隨鈣而增加，但當新生骨組成接近氫氧基磷灰石時，則含量低於偵測極限值。材料中溶解的矽可刺激第一型膠原蛋白合成及造骨細胞分化；體外模擬浸泡研究發現矽酸鹽離子可提供磷灰石成核的有利位置。因此矽酸鈣骨泥將會開啟自身硬化生物活性填充材料新局面。 當以常用的鈣磷酸鹽骨泥(CPC)糊狀物植入骨缺損區開始硬化時，可能會因血液或其他液體而崩解或沖蝕，因此，添加某些黏著提昇因子(cohesion promoters)到骨泥以發展抗沖蝕材料，受到眾多矚目。就我們所知，目前並沒有抗沖蝕的自身硬化及高生物活性矽酸鈣骨泥材料。 本三年計劃的主要目標是結合天然高分子及矽酸鈣，開發出抗沖蝕的自身硬化及高生物活性仿生彈性鈣矽骨泥。第一年計劃將經由各種材料基本分析、體外模擬測試及細胞相容性評估，達成矽酸鈣骨泥製程參數最佳化，製程參數包括溶膠-凝膠法的前導物比例、熟化時間及煅燒溫度等。第二年將利用三種不同細胞研究此具潛力的材料之刺激骨細胞增殖、分化及其基因表現。最後一年將天然高分子幾丁聚醣及明膠加入鈣矽骨泥以改善黏著性與有利臨床操作，達成開發新式的抗沖蝕鈣矽骨泥系統。本三年計畫兼顧基礎研究及醫學應用，預期此三年研究結果在生醫材料及生物科技領域上會有其顯著效能。骨水泥鈣矽化物有機-無機混合物高分子生物活性骨修補Bone cementCalcium silicateOrganic-inorganic hybridBiopolymerBioactivityBone repairProject