2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37515JC virus, a human polyomavirus, causes a demyelinating disease of CNS termed progressive multifocal leukoencephalopathy (PML) by lytically infecting human glial cells. The incidence of PML has increased markedly owing to the prevalence of AIDS. JC virus is also found to induce tumors in inoculated animals and cause transformation in cultured cell lines. Viral T antigen which binds with cellular p53 and retinoblastoma proteins is responsible for the transformation. The purpose of this proposal is to further investigate the structural proteins of JC virus and to determine their respective role(s) in the virion structure, virion assembly and viral infection. The unique aspects of our proposed investigation include: I. The structural organization of JC virion and viral components will be investigated to determine the location of the structural proteins in the virion structure as determined by the interaction of the major structural proteins VP1 and the minor structural proteins VP2 and VP3, with the genomic DNA. II. Interaction domains and bondings for keeping the integrity of virion structure. III. The interactive domains on virion surface will be investigated to identify the viral attachment domain and hemagglutination domain by using epitope mapping and site-directed mutagenesis. IV. Investigation of the post-translational modifications on the structural proteins and identification the modification domains. V. Identification and isolation the cellular receptor for JC virus infection.JC病毒屬於人類多瘤性病毒之一，它會溶解性地感染人類腦細胞而引起進行性多病灶腦白質症(PML)而導 致病人死亡。由於近年來愛滋病的盛行，使得因PML而死亡的病例也隨之增加。JC病毒的大腫瘤抗原與細 胞內p53及RB蛋白結合為該病毒致癌的主要機轉。JC病毒已被證實會引發培養的細胞株癌化及導致動物體 的腫瘤產生。關於JC病毒與人類腫瘤的關係正被積極地研究當中。本研究計畫的目標乃希望更深入地探討 JC病毒的溶解性感染機轉，包括1) JC病毒殼體的結構組織；2) 維持病毒殼體完整性的鏈結力量及可能的鍵 結部位；3)病毒表面與細胞結合分子、與紅血球結合分子以及與中和性抗體結合的抗原決定部位；4)病毒 蛋白在細胞內修飾的研究；5)細胞表面接受體的分離及鑑定。人類多瘤性病毒JC病毒溶解性感染Human PolyomavirusJC VirusLytic InfectionProject