許立松Li-Sung Hsu鍾譯萱Yi-Hsuan Chung生化微生物免疫研究所2026-10-299999-12-3120202020-07-20https://handle.ncl.edu.tw/11296/gy895mhttps://scholars.csmu.edu.tw/handle/123456789/36538膠質母細胞瘤(GBM)是大腦中最普遍的原發性惡性腫瘤，擁有生存率低和復發率高的特性，至今仍沒有有效的治療方法。中草藥中的異黃酮已被證明可以抑制多種癌症細胞的生長，鳶尾黃素是從射干中提取的有效成分並具有多種生物學功能，例如：抗氧化、抗發炎和抗腫瘤。因此本研究目的在探討鳶尾黃素是否對GBM發揮抗腫瘤作用以及潛在分子機轉。結果顯示透過MTT assay證實鳶尾黃素以劑量和時間依賴性顯著抑制GBM細胞的生長，藉由流式細胞儀證實200和300 μM的鳶尾黃素處理24小時可使細胞週期停滯在G0 / G1期，然後透過western blot表明鳶尾黃素增加了磷酸化ERK和p21，並降低了CDK4的蛋白表現量，此外GBM細胞暴露於鳶尾黃素48小時會誘導細胞凋亡，依濃度成比例地降低了PARP蛋白的表達水平，最後使用Boyden chamber證明低濃度鳶尾黃素可以抑制GBM細胞遷移與侵襲的能力，之後藉由Zymography證實隨著藥物濃度上升，MMP-9的活性也隨之下降。總結以上實驗結果顯示鳶尾黃素可以抑制GBM細胞存活率，藉由活化ERK誘導細胞週期的停滯和產生細胞凋亡的現象，也能透過降低MMP-9活性來抑制細胞遷移與侵襲，在此我們認為鳶尾黃素對於GBM有抗腫瘤作用，因此可以作為治療GBM的有效方法之一。Glioblastoma (GBM) is the most common primary malignant tumor in the brain. It is associated with poor survival and high recurrence, and there is still no effective treatment. Flavonoids in traditional Chinese medicines have been shown to inhibit the growth of many cancers. Tectorigenin is an effective component extracted from Belamcanda chinensis and exhibits several biological functions such as: anti-oxidant, anti-inflammation and anti-tumor. Therefore, the aim of the present study was to examine the anti-tumor effect of tectorigenin on GBM and its underlying molecular mechanism. MTT assay indicated that tectorigenin significantly inhibited GBM cells growth in a dose- and time-dependent manner. Flow cytometry confirmed that treatment with tectorigenin at concentration of 200 and 300 M for 24 h induced cell cycle arrest in the G0 / G1 phase. Then, western blot analysis indicated that tectorigenin increased the phosphorylation level of ERK and p21 and decreased the protein expression of CDK4. Exposed to tectorigenin for 48 h induced apoptosis in GBM cells. In addition, tectorigenin reduced the expression level of PARP protein in proportion to tectorigenin concentration. Moreover, low concentration of tectorigenin inhibited the migration and invasion of GBM cells as evidenced by Boyden chamber. Zymography confirmed that tectorigenin decreased the activity of MMP-9 in a dose-dependent manner. Taken together, tectorigenin inhibited GBM cell growth, induced cell cycle arrest and apoptosis by activating ERK, and inhibited cell migration and invasion by reducing MMP-9 activity. We proposed tectorigenin had anti-tumor effects on GBM, so it could be used as one of the effective methods for treating GBM.鳶尾黃素膠質母細胞瘤細胞週期細胞遷移細胞侵襲tectorigeninglioblastoma multiformecell cyclemigrationinvasionthesis