YU-HSIANG KUANYI-CHING LIFU-MEI HUANGYU-CHAO CHANG2026-10-162026-10-162012-07-01Pure ID: 15261309Pure UUID: b6814a23-08d8-455f-8113-7b8c8448d4ad10.1111/j.1365-2591.2012.02017.xhttps://www.scopus.com/pages/publications/84862817919https://scholars.csmu.edu.tw/handle/123456789/29933Aim To evaluate the expression of tumour necrosis factor-α and surface antigens by bisphenol A-glycidyl-methacrylate (BisGMA) on murine macrophage cell line RAW264.7. Methodology Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)-α was analysed by enzyme-linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni's t-test for multigroup comparisons. Results BisGMA exhibited cytotoxicity to RAW264.7 in a dose-dependent manner (P<0.05) during 2-h incubation period. BisGMA was found to increase TNF-α secretion in a dose-dependent manner (P<0.05). In addition, CD11, CD14, CD45, CD54, CD40, CD80, and MHC II were significantly stimulated by BisGMA in a dose-dependent manner (P<0.05). However, MHC I expression was not affected by BisGMA (P>0.05). Conclusions Taken together, the ability of macrophages to induce an appropriate immune response when exposed to BisGMA has the potential to upregulate TNF-α production and expression of surface antigens.8enjournal article