2026-11-092026-11-09https://scholars.csmu.edu.tw/handle/123456789/37776Hibiscus sabdariffa Linne (Malvaceae), an attractive plant believed to be native to Africa is cultivated in Sudan and in eastern and Taiwan. It has been reported to contain a number of flavonoids including gossypetin, hibiscetin and quercetin. In vitro experimental studies have shown that administration of the aqueous extract produces anti-inflammation, hypotensive and hypocholesterolemic. In spite of the wide use of H. sabdariffa extract (HSE) in folk medicine for treatment of various diseases, there are no reports in the literature on their anti-atherosclerosis activity. The aim of this study was to evaluate the effects of an aqueous extract obtained from Hibiscus sabdariffa (HSE) on the (1) susceptibility of LDL to oxidation and on plasma lipid in rabbits affected by experimental atherosclerosis induced by the intake of high levels of saturated fat and cholesterol; (2) inhibition of ox-LDL-induced apoptosis in cultured macrophage, the mechanisms of anti-apoptosis of HSE in macrophage would be studied to determine whether the apoptotic signals and gene expression are involved; (3) inhibition of proliferation and cell cycle progression and induced cell apoptosis in aortic smooth muscle cells (ASMC). The molecular mechanisms of the anti-proliferative and apoptotic effects of HSE in ASMC would be investigated. In the first year, we have found that HSE inhibit Cu++ -mediated LDL-oxidation, apo-B fragmentation and cholesterol degeneration in LDL (Figs. 1-4 and tables 1-2). Furthermore, we found that HSE inhibit plasma triglyceride, cholesterol and arthoscleotic lesions in cholesterol fed rabbit (Fig. 5- 8). In the second years, we found that HSE inhibit ox-LDL-mediated macrophage apoptosis and PARP cleavage (Fig. 9-16). The mechanisms of antiapoptosis in ox-LDL-mediated apoptosis are now in progress. In the third year, we plant to study the mechanisms of the action of HSE on the inhibition of ASMC proliferation. The results of these studies may be used to demonstrate the effects of HSE on the preventive cardiovascular diseases by (1) antilipidemia and inhibit LDL-oxidation; (2) inhibit ox-LDL-induced apoptosis in macrophage; (3) anti-proliferation in ASMC. These results also provide HSE as a health food for preventing cardiovascular disease.洛神花盛產於熱帶地區及台灣東部與南部，具抗血壓及抗發炎等功效，由於其成份含有原兒茶酸及類黃酮素（包括gossypetin, hibiscetin 及quercetin等）都為強抗氧化劑，且都含於水萃取物中。因此本研究探討對（1）洛神花水萃取物（HSE）降血脂及抑制低密度脂蛋白氧化作用，進一步探討（2）抑制氧化型低密度脂蛋白（ox-LDL）誘導macrophage apoptosis（可能與atherosclerotic pathogenesis 有關）及（3）探討抑制 aortic smooth muscle cell （ASMC）增生之作用是否與cell cycle arrest 及apoptosis 有關。上二年度的計劃中，我們發現HSE抑制LDL氧化作用，包括抑制apo B的分解及LDL中膽固醇的degeneration，進一步發現HSE抑制cholesterol及lard oil 餵食10週之兔子血中三酸甘油脂及膽固醇也顯示HSE抑制cholesterol fed 之兔子產生之atheroscleotic lesion。由於ox-LDL誘導 macrophage apoptosis，可能與atherosclerosis或thrombosis相關，因此第二年我們發現討HSE抑制ox-LDL-induced apoptosis及PARP斷裂，其中相關的機轉仍進一步探討中；第三年的工作，我們擬探討HSE對CD-36表現之抑制及對ASMC細胞抗增生，抑制細胞週期，促進凋謝死亡及相關的分子機轉。從以上的結果，我們預期HSE有（1）降血脂、抗LDL氧化作用；（2）抑制ox-LDL-induced atherosclerosis是經由anti-apoptosis in macrophage；（3）抑制ox-LDL receptor CD-36表現;；（4）抑制ASMC細胞增生是經由HSE造成cell cycle arrest 及apoptosis。因此，從以上的研究，HSE可發展為一種預防心血管疾病之保健食品。洛神花水萃取物氧化型低密度脂蛋白降血脂作用凋謝死亡抑制增生Hibiscus sabdariffa extractLDL oxidationhyperlipidemiaapoptosiscell cycle arrestantiproliferationProject